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DNA OLIGONUCLEOTIDE WHICH IS SPECIFIC FOR MELOIDOGYNE SPECIES, DNA VECTOR AND HOST CELL CONTAINING THE OLIGONUCLEOTIDE, USE AND KIT
DNA OLIGONUCLEOTIDE WHICH IS SPECIFIC FOR MELOIDOGYNE SPECIES, DNA VECTOR AND HOST CELL CONTAINING THE OLIGONUCLEOTIDE, USE AND KIT
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机译:DNA寡核苷酸,特异于蛋壳菌种,DNA载体和含有寡核苷酸的宿主细胞,用途和试剂盒
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摘要
The invention relates to a DNA oligonucleotide which is specific for Meloidogyne species, which DNA oligonucleotide has the following sequence: (a) a DNA sequence chosen from the group consisting of SEQ ID No. 1 to SEQ ID No. 20, or (b) a DNA sequence which under conditions of stringency hybridises with a sequence according to (a) and which is specific for Meloidogyne species. Furthermore, the invention relates to a DNA vector containing such a DNA oligonucleotide, as well as to a host cell containing such vector. The invention also relates to a process for determining the presence of a Meloidogyne species, according to which DNA is isolated from a sample to be analysed, and is subsequently subjected to a PCR reaction, using a DNA oligonucleotide according to the invention. Finally, the invention relates to a kit for determining the presence of a Meloidogyne species.
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机译:本发明涉及一种对根结线虫物种具有特异性的DNA寡核苷酸,该DNA寡核苷酸具有以下序列:(a)选自SEQ ID No.1至SEQ ID No.20的DNA序列,或(b)该DNA序列在严格条件下与根据(a)的序列杂交,并且对根结线虫物种具有特异性。此外,本发明涉及含有这种DNA寡核苷酸的DNA载体,以及含有这种载体的宿主细胞。本发明还涉及确定根结线虫种类的存在的方法,根据该方法,从待分析的样品中分离DNA,然后使用根据本发明的DNA寡核苷酸进行PCR反应。最后,本发明涉及一种用于确定根结线虫物种的存在的试剂盒。
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