Identification of Brugia malayi in Vectors with a Species-Specific DNA Probe on Successful Resuscitation of a Patient with Renal Failure

摘要

We evaluated the potential value of a cloned sequence of genomic DNA of Brugia malayi as a species-specific probe. Clone pBm.15 reacted with all stages of 8 different geographic isolates of B. malayi and cross-hybridized with microfilariae of B. timori. It did not hybridize with Wuchereria bancrofti or with B. pahangi, W. kalimantani, Dirofilaria repens, breinlia booliati or cardiofilaria species, animal filariids that can be sympatric with B. malayi. p32-labeled clone pBm 15 correctly indentified mosquitoes infected even with 1 infective larva of B. malayi. This specific DNA probe should be an invaluable tool to monitor control programs of Brugian filariasis.