Mutant epoxide hydrolases

摘要

Mutant epoxide hydrolases having an amino acid substitution of a tyrosine residue in the cap domain of the wild type epoxide hydrolase, in particular mutant epoxide hydrolases having an amino acid substitution of the tyrosine residue corresponding to Tyr152 or Tyr215 in an epoxide hydrolase with an aminoacid sequence according to SEQ ID No. 1. Preferably the Tyr is substituted by Phe. The invention also relates to nucleic acid sequences encoding such mutant epoxide hydrolases expression constructs wherein such nucleic acid sequence according is operably linked to a regulatory region capable of directing the expression of the mutant epoxide hydrolase in a suitable expression host, vectors capable of transforming a host cell characterized in that the vector containing such expression construct, transformed host cells transformed with such a vector, processes for the preparation of such mutant epoxide hydrolases and processes for the preparation of optically active epoxides and/or diols using such mutant epoxide hydrolases.