MUTANT EPOXIDE HYDROLASES FROM MUGIL CEPHALUS

摘要

The invention stems shove consisting of pushing (Mugil cephalus) derived from epoxides from hydrolases, phosphorus phenylalanine 193 by a point mutation amino acids is substituted with tyrosine, SEQ ID NO: 3 in the amino acid sequence of the amino acid sequence of SEQ ID NO: 1 epoxide side-hydrolase; Or shoving of the amino acid sequence of SEQ ID NO: 1 (Mugilcephalus ) Derived from epoxides from the hydrolase, the 193 by point mutations amino acids phenylalanine (Phe, F) is replaced by tyrosine (Tyr, Y), 200 amino acids of the tryptophan (Trp, W), a leucine (Leu, L ) to be substituted, and 378 amino acids glutamate (Glu, E) the aspartate (Asp, D) the hydrolytic activity comprising the mutant amino acid sequence containing the SEQ ID NO: 7 substituted with improved epoxide hydrolase and encoding the gene provides for. ; Epoxide Hydrolase, point mutations, shove