STAINING AGENT AND STAINING METHOD FOR JUVENILE MUGIL CEPHALUS, AND APPLICATION OF ALIZARIN RED IN STAINING AND LABELING OF JUVENILE MUGIL CEPHALUS

摘要

#$%^&*AU2020100372A420200910.pdf#####ABSTRACT The present invention provides a staining agent and staining method for juvenile Mugil cephalus, and application of alizarin red in staining and labelling of the juvenile Mugil cephalus, 5 belonging to the technical field of tracking and monitoring of proliferation and release. Alizarin red has high solubility and a good labeling effect. After impregnation staining of the juvenile Mugil cephalus, good labeling is achieved on both scales and fin spines, and the total labeling rate in fluorescence detection of all samples reaches 100%. After temporary culture for 40 d, the labeling retention rate is 100%, and thus the labeling retention effect is good. In addition, J staining and labeling the juvenile Mugil cephalus by employing alizarin red is simple in operation, can obtain a high-quality labeling effect, and can ensure the survival rate of the juvenile Mugil cephalus in the labeling process.