The invention concerns a method comprising the following steps which consist in: a) causing the chromosomic DNA of the of the Thermococcus hydrothermalis strain to be digested by a restriction enzyme; b) recuperating the resulting fragments by electroelution; c) ligating each of the two types of fragment (500-1500 pb and 1500-9000 pb) in the PYME 1 and PYME 2 plasmids; d) transforming a Saccharomyces cerevisiae yeast strain; e) causing the transformed yeast cells to grow in a medium wherein only those transformed by a plasmid containing the gene coding for an α-glucosidase develop; f) recuperating the cells selected at step e), cultivating them and recuperating the excreted glucoamylase enzyme. The invention is applicable in particular in the starch industry.
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