The invention relates to a method for determining the apolipoprotein-E-polymorphism in a human sample by PCR-amplification of a selected base sequence and detection of the apolipoprotein-E-allele, using a multiplex PCR with 6 to 7 different specific primers, four of the primers in each case ending directly at the mutation-specific sites. The primers are characterised inter alia by base mismatches at the third to fifth site before the 3' end. The invention is also characterised in that the mismatch sites of ELEMENT II and ELEMENT III or ELEMENT V and ELEMENT VI are displaced in relation to the 3' end in order to improve the amplification selectivity.
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