A RECEPTOR TYROSINE KINASE TARGET PROTEIN cDNA CLONING METHOD AND hGRB PROTEINS

摘要

the invention relates to a method for cloning and expression, which allows detection and identification of proteins capable of binding to the tyrosine phosphorylated domains of the receptor tyrosine kinases and has for this purpose a new probe.the probe comprises an amino acid sequence derived from the molecular receptor phosphorylated by tyrosine or a functional derivative thereof, that has at least one tyrosine phosphorylated continues, it is free from the part of the receptor tyrosine kinase and the the marked detected.the invention also describes a process for preparing the probe, a method to locate on the chromosome a gene encoding a protein capable of binding to the tyrosine phosphorylated domains of receptor tyrosine kinases, as well as a process for the purification of such a protein with the probe.both proteins are also described, which has been found to use the process of cloning, protein - 1 and - 2. still, a dna encoding the protein, as well as the methods of detection of these proteins.