Cloning of cDNA for Two Glutamate Receptor Proteins

摘要

The studies described in the original proposal to the ARO were focused on the useof the immunochemical tools developed in these laboratories to identify the glutamate and CPP-binding protein cDNA's and clone these two cDNA's. The aims of these studies included the sequencing of the cloned cDNA's, the expression of the proteins in various cell Systems such as COS and CV-1 cells, and measurement of ligand binding or ion channel properties of these receptor-related proteins. It was anticipated that following the successful completion of these studies, the cDNA libraries would be screened again in order to identify either homologous clones or clones for some of the other subunits of the complex of proteins isolated in these laboratories. This complex of proteins was proposed to form a glutamate/N-methyl-D-asparata (NMDA) receptor-ion channel. Part of the work proposed was focused on the documentation of such receptor activity being associated with the isolated complex of the proteins. The results described in this final report indicate that all of the goals of the proposed research were completed and that additional findings were documented that strongly support the hypothesis that the isolated proteins form a type of glutamate/NMDA receptor.