Freezing Composition of Immature Oocytes and Freezing and Thawing Methods of Immature Oocytes

摘要

The present invention relates to a freezing composition of immature follicles, and to a method of freezing and thawing immature follicles using the same, more specifically, to preserve the magnetic reproductive cells such as livestock, conventional livestock, rare livestocks having high ability genetic factors Freezing composition of immature follicle eggs and the method of freezing and thawing immature follicle eggs using the same to promote the preservation and utilization of genes,;In the present invention, 10% (1.8Mol) Ethylene glycol, 5% Ficoll, and 1.7% (0.05M) in a mixture of 5% serum in Tissue Culture Medium 199 or Phosphate Buffer Slaine or a known culture medium used for the culture of fertilized eggs. ) It is characterized by the effective freezing of frozen premature oocytes by freezing composition by additional mixing of trehalose and the use of immature oocytes by fusing frozen premature oocytes when necessary.