MUTANT CANDIDA TROPHICALIS SDB-101 AND PREPARATION METHOD OF WYLITOL THEREBY

摘要

The present invention relates to a novel microorganism and a method for producing xylitol using the same, and to a method for producing a high yield of xylitol from xylose using a novel mutant strain, Candida Tropicalis SDB-101.;There are two methods for producing xylitol, which is used as a sweetener, but there are chemical methods and methods using microorganisms.However, chemical methods are difficult to purify, low yield, risk and waste problems. This inexpensive and easy separation and purification process, but still low yield, the present invention was invented to solve this conventional problem.;The present invention is to produce a mutant strain producing high yield of xylitol from xylose and to produce xylitol in high yield using the same. In the present invention, the mutant strain Candida Tropicalis SDB-101 is the first selection of a fast growth strain using a sample of sludge from the xylose company, and the medium consisting of xylose, yeast extract, potassium monophosphate After selecting the strain with high yield of xylitol at 2nd time and spreading it in YM medium added with NTG, the resulting colonies were separated again and repeated twice or more, and the resulting mutant strains were smeared on YM medium containing xylose and colonies. After isolation again, a strain with high yield and high heat resistance is selected. The mutant strain is cultured in a YM medium and then cultured in a fermentation medium having xylose to obtain a high yield of xylitol.