CDNA ENCODING A HUMAN C6 BETA-CHEMOKINE, LEUKOTACTIN-3, AND A PROCESS FOR PREPARING A RECOMBINANT LEUKOTACTIN-3

摘要

The present invention utilizes a cDNA encoding a novel protein (leukotactin-3: Lkn-3) belonging to C6 β-chemokine isolated from a human mononuclear cell line (monocytic cell line), an expression vector comprising the same, an electric expression vector The present invention relates to a method for producing an electrical protein that attracts subsets of peripheral blood leukocytes. The open reading frame of Lkn-3 cDNA of the present invention encodes a total of 120 amino acids including a signal peptide consisting of 21 amino acids and a mature protein consisting of 99 amino acids. Recombinant Lkn-3, which was purely isolated from the expression of recombinant Lkn-3 cDNA in recombinant E. coli or insect cells, significantly inhibited colony formation, chemotaxis that attracted lymphocytes, monocytes, and neutrophils, and hMIP-1α and RANTES It has been shown to bind to the receptor of binding CCR1. The recombinant Lkn-3 protein can be used for antibody production, treatment during HIV-1 infection, bone marrow hepatocyte protection during chemotherapy or radiation therapy, leukemia inhibitor, and the like.