POLYCLONAL ANTIBODY AND ELISA SYSTEM FOR DETECTION AND IDENTIFICATION OF PSEUDOMONAS TOLAASII

摘要

PURPOSE: A measurement of enzyme immunity to detect pseudomonas tolaasii in an agaric mushroom is provided to achieve a faster and more convenient detection of the degree or level of contamination by bacteria causing cercospora iythracearum. CONSTITUTION: The noncompetitive indirect enzyme immunity measurement to detect pseudomonas tolaasii is first carried out by coating a tissue and a sample having pseudomonas tolaasii with tris-HCl; cultivating pseudomonas tolaasii bacilli as bacterial brown blotch from agaric mushroom; adding an anti-pseudomonas tolaasii polyclonal antibody obtained from an animal; and adding an anti-rabbit enzyme zygous and substrate solution to cause an enzyme reaction.