Antibody/antigen detection system with application to high throughput identification and screening. Final report

摘要

This report focuses on the design of an imaging system capable of reading fluorescing activity from individual grid cells contained on a biosensor grid array cartridge. Each cell on the cartridge shall be designed to facilitate covalent binding of antigen/antibody bioreactive components. The imaging system shall include the image capture electronics, the video analog to digital conversion, and the image processing and analysis software. The software shall be sufficient to store the raw data and extract information from the data, such as unique identify recognition from the fluorescing pattern generating on the grid cartridge. The imaging system shall have the ability to distinguish the on/off condition of each grid cell as well as intensity determination with an accuracy of 8-bit gray scale discrimination.