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A method for detecting a nucleic acid by means of a nukleinsaeuresonde, which is a specific capture and a detection allows all at once
A method for detecting a nucleic acid by means of a nukleinsaeuresonde, which is a specific capture and a detection allows all at once
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机译:一种用于通过核酸核糖核酸酶检测核酸的方法,该方法是特异性捕获,并且检测允许一次全部检测
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摘要
A nucleic acid detection assay is disclosed which assay comprises hybridizing a large nucleic acid probe (i.e. preferably more than 100 bp long) with a RNA or DNA target nucleic acid present in a biological sample. The probe in this method is to comprise both a detection element and a capture element and is to to be degradable by a suitable enzyme when it is single stranded. Following hybridization the sample is treated with a enzyme capable of degrading unhybridized nucleic acids. Then the hybrids are captured by contacting them with a solid support comprising an element capable of binding with the capture element of the probe. Finally the detection element of the probe is used to detect the probe, thereby indicating that the target nucleic acid is present in the biological sample.
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