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Use of N-acetylcysteine to store red blood cells and a method of aging red cells with nitrogen
Use of N-acetylcysteine to store red blood cells and a method of aging red cells with nitrogen
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机译:N-乙酰半胱氨酸用于储存红细胞的方法以及用氮气老化红细胞的方法
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摘要
The invention provides a method of preserving red blood cells. The method comprises treating the red blood cells with about 2 mM to about 35 mM of N-acetylcysteine. The invention further provides a method of aging red blood cells. This method comprises isolating red blood cells; incubating the isolated red blood cells at about 37° C.; and cyclically exposing the incubated red blood cells to N.sub.2 for about 15 minutes then to ambient air for about 5 minutes for a total of about 16 hours. This method allows for the timely screening of agents for their ability to affect the premature aging of red blood cells. This timely screening can be conducted in the course of 16 hours as opposed to weeks or months that would be necessary if the red cells could not be prematurely aged in accordance with the method of the subject invention.
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