Method for producing transgenic cucumber that produces high levels of superoxide dismutase

摘要

The invention relates to a transgenic plant that produces high levels of superoxide dismutase (SOD) and to a method for producing the transgenic plant. The hypocotyl section of seedlings is co-cultured with Agrobacterium transformant and regenerated by adventitious shoot induction and by root induction, where the Agrobacterium transformant contains an expression vector that comprises the promoter of a fruit- dominant ascorbate oxidase gene, an SOD gene isolated from cassava, and an herbicide-resistant bar gene. The present invention also relates to a method for inducing adventitious shoot from hypocotyl sections in plant tissue culture, thus providing a method for the efficient production of transgenic plants maintaining higher SOD activity in fruits. Therefore, the SOD transgenic cucumber of the present invention can be used for cosmetics, additives in functional foods, and medicines as well as having tolerance to herbicides and environmental stresses.