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Method for producing transgenic cucumber that produces high levels of superoxide dismutase

机译:产生高水平超氧化物歧化酶的转基因黄瓜的生产方法

摘要

The present invention relates to transgenic plants that produces high levels of superoxide dismutase (SOD) and to methods for producing said transgenic plants. More particularly, the present invention relates to a transgenic plant and a method, in which the hypocotyl section of seedlings is co-cultured with Agrobacterium transformant and regenerated by adventitious shoot induction and by root induction, where saidAgrobacterium transformant contains an expression vector which comprises the promoter of fruit-dominant ascorbate oxidase gene, SOD gene isolated from cassava, and herbicide-resistant bar gene. The present invention also relates to a method for inducing adventitious shoots from the hypocotyl section in plant tissue culture, thus providing a method for the efficient production of transgenic plants maintaining higher SOD activity in fruits. Therefore, the SOD transgenic cucumber in the present invention can be used as a material for cosmetics, additives in functional foods, and medicines as well as a substance which has tolerance to herbicides and environmental stresses.
机译:本发明涉及产生高水平超氧化物歧化酶(SOD)的转基因植物,并涉及产生所述转基因植物的方法。更具体地,本发明涉及转基因植物和方法,其中将幼苗的下胚轴部分与农杆菌转化体共培养并通过不定芽诱导和根诱导再生,其中所述< I>农杆菌转化体含有表达载体,该表达载体包含以水果为主的抗坏血酸氧化酶基因,从木薯中分离的SOD基因和抗除草剂的bar基因的启动子。本发明还涉及在植物组织培养中从下胚轴部分诱导不定芽的方法,从而提供有效生产保持果实中较高SOD活性的转基因植物的方法。因此,本发明的SOD转基因黄瓜可用作化妆品,功能性食品和药物中的添加剂以及对除草剂和环境胁迫具有耐受性的物质。

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