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Method for producing transgenic cucumber that produces high levels of superoxide dismutase
Method for producing transgenic cucumber that produces high levels of superoxide dismutase
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机译:产生高水平超氧化物歧化酶的转基因黄瓜的生产方法
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摘要
The present invention relates to transgenic plants that produces high levels of superoxide dismutase (SOD) and to methods for producing said transgenic plants. More particularly, the present invention relates to a transgenic plant and a method, in which the hypocotyl section of seedlings is co-cultured with Agrobacterium transformant and regenerated by adventitious shoot induction and by root induction, where saidAgrobacterium transformant contains an expression vector which comprises the promoter of fruit-dominant ascorbate oxidase gene, SOD gene isolated from cassava, and herbicide-resistant bar gene. The present invention also relates to a method for inducing adventitious shoots from the hypocotyl section in plant tissue culture, thus providing a method for the efficient production of transgenic plants maintaining higher SOD activity in fruits. Therefore, the SOD transgenic cucumber in the present invention can be used as a material for cosmetics, additives in functional foods, and medicines as well as a substance which has tolerance to herbicides and environmental stresses.
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