Primers and universal probes species-specific, and genus-specific for the identification and rapid detection of antibiotic resistance genes and related fungal pathogens and bacterial common from clinical samples for the diagnosis of microbial laboratory

摘要

(57) [summary] of (i) the test sample, any bacteria, (ii) Enterococcus faecium (Enterococcus faecium), Listeria monocytogenes (Lister monocytogenes), Neisseria Meningichitisu (Neisseria meningitidis), Staphylococcus · seed Sapurofitekasu (Staphylococcus saprophyficus), Streptococcus agalactiae Candida albicans and (Streptococcus agalactiae) of (Candida albicans), and, (iii) Enterococcus (Enterococcus), Neisseria (Neisseria), Staphylococcus (Staphylococcus), Streptococcus ( method using the amplification primers or probes in order to detect the DNA from the genus Candida and Streptococcus) of (Candida), and quantifying the identified based on DNA is disclosed. In the test _sample, bla _tem, bla shv, bla _robb, bla _oxa, blaZ, andB, aacC1, aacC2, aacC3, aac6'-IIa, aacAA, aub (6 '), vanA, vanB, vanC, msrA, satA, In order to detect the antibiotic resistance gene selected from the group consisting of - '(', vat, vga, ermA, cirmB, ermC, mecA, the sul and int 2) aph, identified, and quantified aac (6) ' method using a probe or amplification primers, based on the DNA are also disclosed. The microbial species, genus and resistance genes are those that are clinically meaningful all are those commonly encountered in clinical specimens in various. Measurements based on these DNA is rapid, it is accurate and can be used in clinical microbiology laboratories for routine diagnosis. Diagnosis novel means of these are useful in order to improve the speed and accuracy of diagnosis of microbial infection, I allow the treatment more effective by this. Detection of the genus, detection of (iii) above antibiotic resistance genes and quantitative, and / or identification of species and fungi and / or bacteria (iii) above, and / or quantitative, and universal detection of (i) or bacteria / diagnostic kit for the quantitative, and identification are also disclosed.