PROBLEM TO BE SOLVED: To selectively extract a high-purity glutelin protein, having high safety and useful for a reagent, a substrate, a food, a medicine, etc., in a rice reserve protein by absorbing water in rice, a rice flour or a white rice bran, directly treating the resultant rice, etc., with a dilute acid or drying the rice, etc., and then treating the dried rice, etc., with the dilute acid. ;SOLUTION: Rice, a rice flour or a white rice bran, etc., is immersed in water (at 15°C) in an amount of 10 times based on the rice, etc., for 2 hr to absorb water therein. The resultant rice, etc., is then drained, steamed with a steaming basket for 50min, subsequently allowed to cool or directly used or dried, then immersed in a 0.05-5% solution of lactic acid, malic acid, succinic acid, citric acid, fumaric acid, acetic acid, phosphoric acid and/or hydrochloric acid, etc., and treated with the dilute acid to extract only a protein body II fraction in a rice reserve protein. The resultant extract solution is separated and a 2% sulfosalicylic acid is added thereto to precipitate the protein, which is subsequently collected by centribugation. Thereby, the objective high-purity rice reserve glutelin protein, has high safety and is usable as a reagent, a substrate and further a food or a medicine.;COPYRIGHT: (C)1997,JPO
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