METHODS OF PRODUCING ANTI-ANGIOGENIC PROTEINS: ENDOSTATIN, ANGIOSTATIN OR RESTIN, USING A PICHIA YEAST EXPRESSION SYSTEM

摘要

Methods for making proteins with anti-angiogenic properties are disclosed, including angiostatin, endostatin and restin. The system used is a yeast expression system which produces biologically active proteins at high titer, specifically the Pichia pastores yeast expression system.