首页> 外国专利> A PROCESS FOR CONSTRUCTING DNA BASED MOLECULAR MARKER FOR ENABLING SELECTION OF DROUGHT AND DISEASES RESISTANT GERMPLASM SCREENING

A PROCESS FOR CONSTRUCTING DNA BASED MOLECULAR MARKER FOR ENABLING SELECTION OF DROUGHT AND DISEASES RESISTANT GERMPLASM SCREENING

机译:构建基于DNA的分子标记物以促进抗旱和抗病菌的筛选的方法

摘要

This invention relates to a process for constructing DNA-based molecular markers in plants comprising: identifying and selecting the gene sequences relating to stress from available database and literature; submitting the selected gene sequence for similarity search to obtain other sequences from the database similar to the selected gene sequence; subjecting the sequences obtained from similarity search to multiple alignment; removing redundant sequences if any, to get a data set of proteins involved in biotic and abiotic stress response; picking blocks or motifs from the data set of proteins on basis of statistical significance; subjecting the data set of proteins to Blockmaker to pick the same set of blocks or motifs; analysing the motifs for the functionality.
机译:本发明涉及在植物中构建基于DNA的分子标记的方法,该方法包括:从现有数据库和文献中鉴定和选择与胁迫有关的基因序列;提交选择的基因序列进行相似性搜索,以从数据库中获得与选择的基因序列相似的其他序列;使从相似性搜索获得的序列进行多重比对;删除多余的序列(如果有的话),以获得涉及生物和非生物应激反应的蛋白质数据集;根据统计显着性从蛋白质数据集中选择块或基序;使蛋白质数据集经过Blockmaker挑选相同的块或基序;分析功能的主题。

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