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Process for constructing dna based molecular marker for enabling selection of drought and diseases resistant germplasm screening
Process for constructing dna based molecular marker for enabling selection of drought and diseases resistant germplasm screening
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机译:构建基于dna的分子标记以选择抗旱和抗病种质的方法
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摘要
This invention relates to a process for constructing DNA-based molecular markers in plants comprising: identifying and selecting the gene sequences relating to stress from available database and literature; submitting the selected gene sequence for similarity search to obtain other sequences from the database similar to the selected gene sequence; subjecting the sequences obtained from similarity search to multiple alignment; removing redundant sequences if any, to get a data set of proteins involved in biotic and abiotic stress response; picking blocks or motifs from the data set of proteins on basis of statistical significance; subjecting the data set of proteins to Blockmaker to pick the same set of blocks or motifs; analysing the motifs for the functionality.
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