FUSION GENE OF TREHALOSE SYNTHASE, FUSION ENZYME PROTEIN AND METHOD FOR PRODUCING TREHALOSE USING THE SAME

摘要

PURPOSE: A fusion gene of trehalose synthase, fusion enzyme protein and a method for producing trehalose using the same are provided. The trehalose is effectively produced in higher yield using a fusion gene of BvMTSase and BvMTHase gene that code trehalose biosynthase. CONSTITUTION: The fusion gene is produced by fusing the BvMTSase gene and BvMTHase gene isolated from Brevibacterium helvolum, in which the BvMTSase gene is 2,328bp of Brevibacterium maltooligosyltrehalose synthase gene, and the BvMTHase gene is 1,767bp of Brevibacterium maltooligosyl trehalose trehalohydrolase gene. The method for producing trehalose comprises the steps of: fusing the BvMTSase gene and BvMTHase gene to produce the BvMTSHase gene; inserting the BvMTSHase gene into E. coli expression vector pRSET; transforming E. coli with the transformed expression vector and incubating the transformant for 12 hours; inducing the expression of fusion enzyme by adding IPTG and incubating for 4 hours; and isolating and purifying the fusion enzyme by using Ni2+-NTA-agarose gel and SDS-PAGE; and producing trehalose from soluble starch by using the fusion enzyme BvMTSHase.