11 Bacillus subtilis EK11 strain and process for preparation of protopectinase using the same

摘要

PURPOSE: A Bacillus subtilis EK11 strain and a method for producing protopectinase therefrom are provided, thereby the protopectinase can be mass-produced. CONSTITUTION: The Bacillus subtilis EK11 (KCTC 8986P) strain is isolated by the steps of: treating a mother strain, Bacillus subtilis IFO12113, with nitrosoguanidine for 40 minutes; spreading the mutant strain on an LB agar medium containing 1.5% of arabinogalactan; incubating it and first selecting a strain from colonies forming a transparent ring therearound; incubating the selected strain in a medium containing 1% of glucose, 0.2% of (NH4)2SO4, 1.4% of KH2PO4, 0.6% of K2HPO4, 0.1% of Na3-citrate2H2O, and 0.02% of MgSO4H2O; and second selecting a strain having a high protopectinase productivity by measuring the activity of protopectinase and identifying it as Bacillus subtilis EK11 (KCTC 8986P). The protopectinase is produced by incubating Bacillus subtilis EK11 (KCTC 8986P) in a medium containing 0.7% of corn starch, 0.3% of yeast extract, 1.4% of KH2PO4, 0.6% of K2HPO4, 0.02% of MgSO4 H2O and 0.1% of Na3-citrate2H2O.