Study on the Optimum Process Condition of Feather Fermentation using Bacillus Subtilis Mutation Strains

摘要

According to the optimization experimental results of feather fermentation using the compound mutation strain of Bacillus subtilis which was obtained through fermentation condition of ultraviolet ray and sodium nitrite, This paper presented the optimum fermentation condition was culture temperature 33 °C, culture duration 72 hours, shaker speed 170rpm, inoculums 6%, liquid volume 100mL/500mL, initial pH value of the fermentation medium which was suitable for the growth of the strain 7.0, and the optimal pH value for producing enzyme by the strain was 7.5-8.0. During the 60 hours culture, the highest yielding rate for producing enzyme by the strain was 1.267 U/mL. The decompose rate of the feather after 72 hours was 69.61%. The amino acid concentration in the fermentation medium fluid reached 22.66mg/mL. This research provided the optimum process parameters for producing biological fermentation feather powder in industry.