METHOD FOR PREPARING SILK POWER PEPTIDE BY ENZYMATIC DEGRADATION

摘要

PURPOSE: Provided is a method for preparing silk power peptide by enzymatic degradation of silk fibroin. The peptide has a molecular weight of 12,000-13,000, polymerization of 163 and solubility of 70% at pH 1-13, and its internal absorptivity is thus high, so as to contribute to development of functional foods or medicines. CONSTITUTION: The method for preparing silk power peptide by enzymatic degradation of silk fibroin comprises the following steps of: (a) purifying silk fibroin by adding flavourzyme or a protein produced from Aspergillus oryzae and Bacillus subtilis, Bacillus thermoproteolyticus Rokko thereinto; (b) removing salt by adding calcium chloride and ethanol in the resultant and dialyzing them; and (c) obtaining powdered peptide by adding flavourzyme or a protein produced from Aspergillus oryzae and Bacillus subtilis, Bacillus thermoproteolyticus Rokko into the solution and then reacting and freeze-drying them.