METHOD FOR SEPARATION AND PURIFICATION OF FIBRINOLYTIC ENZYME FROM EXTRACT OF EARTHWORM AND cDNA GENE CODING SAME ENZYME

摘要

PURPOSE: Provided are a method for separation and purification of fibrinolytic enzyme from the extract of Earthworm, and cDNA gene coding the fibrinolytic enzyme. CONSTITUTION: The fibrinolytic enzyme is prepared from the extract of Earthworm by cation-exchange column chromatography; hydrophobic binding chromatography; affinity chromatography using benzamide as a ligand; and affinity chromatography using Lima Bean Trypsin inhibitor as a ligand.