New regulatory sequences from the fascin gene, useful for providing dendritic cell-specific expression of e.g. antigens, e.g. for vaccination against tumors and infections

摘要

Regulatory sequences (A) that provide specific expression in dendritic cells (DC). (A) are; (i) sequences from nucleotides (nt) n to 3069 of a 16951 base pairs (bp) sequence (S72), where n = 1, 1136, 1451, 1621, 1830, 2127, 2410 or 2700; (ii) sequences in clone DSM 13274 produced by amplification with primers (S37) and (m), where m = 36, 38-44; (iii) sequences containing at least a functional part of (i) or (ii), able to provide DC-specific expression; and (iv) sequences that hybridize to (i)-(iii) and provide DC-specific expression. All numbered sequences are reproduced. Independent claims are also included for the following: (1) recombinant nucleic acid (I) containing (A); (2) vector (II) containing (A) or (I); (3) preparing (M) genetically modified host cells (III) by transformation with (II); (4) (III) modified with (A), (I) or (II), or prepared by (M); (5) nucleic acid (A') of at least 15 nt that hybridizes specifically, under stringent conditions, with one strand of (A); (6) antigen-specific stimulation (M1) of T cells in vitro; (7) preparation of pharmaceutical composition from the T cells of (f); (8) preparation of DC able to stimulate T cell; (9) preparation of a pharmaceutical composition containing the DC; and (10) pharmaceutical composition containing (I), (II) or (III), (8) or (9).