New oligonucleotide, comprising specified nucleotide sequences, is argonaute-1 expression inhibitor, useful for diagnosing or treating disorders or diseases e.g. angiogenesis, hematopoiesis, embryogenesis or tumors e.g. breast cancer

摘要

Oligonucleotide, comprising one or more sequences including SEQ ID NOs. 1-3, is new. Oligonucleotide, comprising one or more sequences including (5'-gatcttagggatgtccacctc-3') (SEQ ID NO. 1), 5'-cacctcgtagtggtacacgt-3' (SEQ ID NO. 2) and 5'-gcttgggctgctcatctatg-3' (SEQ ID NO. 3), is new. Independent claims are included for: (1) a vector comprising the oligonucleotide; (2) a nucleic acid construct comprising the oligonucleotide and a detectable marker; (3) a composition comprising the oligonucleotide, optionally in conjunction with one or more adjuvants, diluents and/or carriers; (4) a diagnostic composition comprising the oligonucleotide or the nucleic acid construct; (5) a method for specifically inhibiting the expression of ago-1 in a cell in vitro, comprising introducing the oligonucleotide or the vector into the cell, and providing suitable hybridization conditions; and (6) detecting argonaute-1 (ago-1) mRNA in a cell in vitro, comprising introducing the nucleic acid construct into a cell, providing suitable hybridization conditions and detecting the detectable marker. ACTIVITY : Angiogenesis Inhibitor; Antianemic; Anticonvulsant; Nootropic; Cytostatic. MECHANISM OF ACTION : Argonaute-1 (Ago-1) expression inhibitor. Tests details are described but no results given.