New oligonucleotide, comprising specified nucleotide sequences, is argonaute-2 expression inhibitor, useful for diagnosing or treating disorders or diseases e.g. angiogenesis, hematopoiesis, embryogenesis or tumors e.g. breast cancer

摘要

Oligonucleotide, comprising one or more sequences including SEQ ID NOs. 1-4, is new. Oligonucleotide, comprising one or more sequences including 5'-tattcctgcccccgtagag-3' (SEQ ID NO. 1), 5'-gttttgtgttgctttcactctcag-3' (SEQ ID NO. 2), 5'-gtgttttgtgttgctttcactctc-3' (SEQ ID NO. 3) and 5'-gttttgtgttgctttcactctc-3' (SEQ ID NO. 4), is new. Independent claims are included for: (1) a vector comprising the oligonucleotide; (2) a nucleic acid construct comprising the oligonucleotide and a detectable marker; (3) a composition comprising the oligonucleotide, optionally in conjunction with one or more adjuvants, diluents and/or carriers; (4) a diagnostic composition comprising the oligonucleotide or the nucleic acid construct; (5) a method for specifically inhibiting the expression of argonaute 2 (ago-2) in a cell in vitro, comprising introducing the oligonucleotide or the vector into the cell, and providing suitable hybridization conditions; and (6) detecting ago-2 mRNA in a cell in vitro, comprising introducing the nucleic acid construct into a cell, providing suitable hybridization conditions and detecting the detectable marker. ACTIVITY : Angiogenesis Inhibitor; Antianemic; Anticonvulsant; Nootropic; Cytostatic. MECHANISM OF ACTION : Argonaute-2 (Ago-2) expression inhibitor. The ability of the oligonucleotide to inhibit human Ago-2 mRNA expression was tested in vitro using human bladder carcinoma cell line (ECV304). The result showed that the oligonucleotide (asON 3024/24 having SEQ ID NO: 4) exhibited IC 50value of 15 nM.