Nucleic acid sequences from alkane metabolizing Candida yeast, encoding cytochrome b5 and used for the oxidation of long chain alkyl compounds and for the production of long chain dicarbonic acids

摘要

Nucleic acid sequences from alkane metabolizing Candida yeasts, encoding cytochrome b5-polypeptide, its fragments, variants and mutations, are new. Independent claims are also included for the following: (1) a cytochrome b5-polypeptide encoded by the novel nucleic acid; (2) cytochrome b5-polypeptide comprising a 126 residue amino acid sequence, fully defined in the specification, its variants with deletions or modifications, which have cytochrome b5-activity; (3) vectors comprising the novel nucleic acids; (4) host cells comprising the vector of (3); (5) antibodies specific for the cytochrome b5-polypeptide; (6) use of the nucleic acid or the resulting polypeptide for stimulating the activity of cytochrome P450-enzymes.