Method for repopulating human bone marrow comprising culturing CD34amp;plus; cells in a serum free medium

摘要

A serum-free medium which supports the proliferation and differentiation of CD34^+ cells purified from normal bone marrow, peripheral blood of patients treated with cytokines, and umbilical cord blood is described. The recipe for the formulation is given, which provides a medium suitable for the proliferation and differentiation of CD34^+ cells for use in human therapeutic protocols. The cells CD34^+ cultured in a the medium of the present invention are used in a method for repopulating human host bone marrow.