Autologous serum-added bone marrow cell culture system, autoserum-added bone marrow cell culture method, and method for producing a pharmaceutical composition comprising autologous serum-added bone marrow cells as an active ingredient

摘要

PROBLEM TO BE SOLVED: To prepare bone marrow cells collected from a subject without using an anticoagulant, in a liquid-tight state using a culture medium, and by culturing the serum of the subject prepared in the liquid-tight state. Furthermore, an autoserum-added bone marrow cell culture system and an autoserum-supplemented bone marrow cell culture method for culturing and a method for producing a pharmaceutical composition containing autologous serum-added bone marrow cells as an active ingredient are provided. An autoserum-added bone marrow cell culture system for culturing bone marrow cells collected from a subject without using an anticoagulant with the subject's serum, comprising a bone marrow cell suspension storage device, A blood collection storage device, an autoserum acquisition device, and a bone marrow cell culture device. According to the present invention, bone marrow cells can be cultured cleanly, safely, reliably and efficiently in the field of regenerative medicine related to treatment of cranial nerve diseases such as cerebral infarction, spinal cord injury, and demyelinating disease.