Method for producing microbulbs of garlic amp;lcub;Allium sativum l.amp;rcub; in vitro

摘要

A method for producing the bulbs of Garlic with saving the cost for producing them and enhancing the work efficiency and the yield by dark-culturing and/or liquid media-culturing of the garlic tissues in vitro is provided, which comprises the steps of:;a) isolation and excision of the virus-free tissues in the length of 0.2 to 0.3 mm obtained from the meristem of parent body of garlic;;b) inoculating the excised tissues from the meristem tissue of garlic onto the solid-type primary media;;c) culturing the tissues inoculated onto the solid-type primary media under the light condition at 25° C. in the culturing room for 4 weeks;;d) propagating the shoots regenerated from the cultured tissues at the multiplication media for 4 weeks;;e) transferring the propagated shoots into the liquid-type media with additional components of 90 g/l of sucrose and plant growth regulators and culturing them primarily for 10 days;;f) transferring the primarily cultured tissues into the liquid-type media having the same composition as the media used in the step e) with additional components of 140 g/l of sucrose and plant growth regulators;;g) secondarily culturing the said tissues at about 25° C. and under the dark-condition in the culturing room for 6 weeks;;h) harvesting the microbulbs from the virus-free garlic plants in vitro;;in which the steps f) and g) are carried out in the altered liquid-type MS media under the dark-condition with no artificial illumimation.