Micropropagation of Garlic (Allium sativum) by Bulblet Chipping In Vitro

摘要

Garlic (Allium sativum) is well known as one of the noticeable foods which have many medicinal effects for human health from ancient times. In Mie Prefecture, a local cul-tivar 'Iki-wase' traditionally has been cultivated. Recently, it has been shownthat this cultivar has highly effective functions as an antioxidant. Because of this, we decided to develop a rapid and effective propagation method for 'Iki-wase'. Several tissue culture methods have been reported. However, those methods have some problems, such as the occurrence of somatic variation (Luciani et al., 2006), the need for long-term cultivation (Nagakubo et al., 1993), and the necessity of mastering sldllful techniques (Ayabe and Sumi, 1998). In this report, we tried to apply the bulbletchipping method used for nerine (Ezura, 1993) and amaryllis (Yanagawa, 1988) to multiply garlic bulbs rapidly without somatic variations.