Methods of altering the animal genome by joining the movement of site-specific recombination enzyme

摘要

(57) site-specific recombination enzyme in active form [Abstract] The present invention relates to a method of transferring oocytes of mammals, especially the Cre. The recombinant enzyme is in a state that is expressed under the control of a specific promoter in male germ cells with a specific one site, bound to the chromatin of the sperm at least. The method is particularly useful modification of the animal genome, for example, or inserted into the animal genome the foreign sequence, to replace an array of another sequence that is. If the promoter is 1 promoter Sycp, One application, to allow recombination between chromosomes from the mother and paternal. The present invention, animals can be produced by this method, and relates to pharmaceutical, cosmetic and agricultural use in the food industry these transgenic animals.