Site-specific recombination method using a novel site-specific recombination enzyme and its recognition sequence

摘要

A polynucleotide encoding a site-specific recombination enzyme, which comprises any one polypeptide selected from the following polypeptides: (1) a polypeptide comprising the amino acid sequence depicted in SEQ ID NO: 2 or SEQ ID NO: 8; (2) a polypeptide which comprises an amino acid sequence produced by deleting, substituting or inserting one or several amino acid residues in the amino acid sequence depicted in SEQ ID NO: 2 or SEQ ID NO: 8 and which has a site-specific recombination enzyme activity of the polypeptide (1); (3) a polypeptide which comprises an amino acid sequence having a 70% or higher homology with the amino acid sequence depicted in SEQ ID NO: 2 or SEQ ID NO: 8 and which has a site-specific recombination enzyme activity of the polypeptide (1); and (4) a polypeptide which can be recognized by an antibody capable of specifically recognizing a polypeptide comprising the amino acid sequence depicted in SEQ ID NO: 2 or SEQ ID NO: 8 and which has a site-specific recombination enzyme activity of the polypeptide (1).