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NOVEL SITE-SPECIFIC RECOMBINATION ENZYME AND SITE-SPECIFIC RECOMBINATION METHOD USING SEQUENCE CAPABLE OF RECOGNIZING THE ENZYME
NOVEL SITE-SPECIFIC RECOMBINATION ENZYME AND SITE-SPECIFIC RECOMBINATION METHOD USING SEQUENCE CAPABLE OF RECOGNIZING THE ENZYME
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机译:利用识别酶的序列能力的新型位点特异性重组酶和位点特异性重组方法
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摘要
A polynucleotide encoding a site-specific recombination enzyme, which comprises any one polypeptide selected from the following polypeptides: (1) a polypeptide comprising the amino acid sequence depicted in SEQ ID NO: 2 or SEQ ID NO: 8; (2) a polypeptide which comprises an amino acid sequence produced by deleting, substituting or inserting one or several amino acid residues in the amino acid sequence depicted in SEQ ID NO: 2 or SEQ ID NO: 8 and which has a site-specific recombination enzyme activity of the polypeptide (1); (3) a polypeptide which comprises an amino acid sequence having a 70% or higher homology with the amino acid sequence depicted in SEQ ID NO: 2 or SEQ ID NO: 8 and which has a site-specific recombination enzyme activity of the polypeptide (1); and (4) a polypeptide which can be recognized by an antibody capable of specifically recognizing a polypeptide comprising the amino acid sequence depicted in SEQ ID NO: 2 or SEQ ID NO: 8 and which has a site-specific recombination enzyme activity of the polypeptide (1).
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机译:编码位点特异性重组酶的多核苷酸,其包含选自以下多肽的任何一种多肽:(1)一种多肽,其包含SEQ ID NO:2或SEQ ID NO:8所示的氨基酸序列; (2)一种多肽,其具有通过在SEQ ID NO:2或SEQ ID NO:8所示的氨基酸序列中缺失,取代或插入一个或多个氨基酸残基而产生的氨基酸序列,并且具有位点特异性重组多肽的酶活性(1); (3)一种多肽,其包含与SEQ ID NO:2或SEQ ID NO:8所示的氨基酸序列具有70%或更高同源性的氨基酸序列,并且具有该多肽的位点特异性重组酶活性( 1); (4)可以被能够特异性识别包含SEQ ID NO:2或SEQ ID NO:8所示的氨基酸序列的多肽的抗体识别的多肽,该多肽具有该多肽的位点特异性重组酶活性。 (1)。
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