Methods for producing heterologous Poribepuchido deletion in filamentous fungal mutant cells - trichothecenes

摘要

(57) SUMMARY The present invention, cultured under conditions conducive to the production of the polypeptide variant of (a) a parent filamentous fungal cell, (i) the variant, the polypeptide where (ii) the mutant is cultured under the same conditions, and comprises a second nucleic acid sequence comprising a modification of at least one gene of a gene involved in the production of trichothecenes and first nucleic acid sequence encoding If, generates less trichothecene than the parent filamentous fungal cell; relates to a method for producing comprising placing isolating the polypeptide (b) to the culture medium, the polypeptide and. The present invention also relates to a method for obtaining the mutant cells and mutant filamentous fungal cell. The present invention also, I also relates to isolated nucleic acid sequences encoding the diene synthase and Toriko Toriko diene synthase isolated. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequence, and also relates to a method for synthesizing Trichoderma synthase. The present invention also relates to a process for for obtaining a mutant cell such and use and mutant cells, comprising a marker-free modification of the gene.