METHOD FOR PRODUCING FRACTION HAVING HIGH MILK-DERIVED BASIC CYSTATIN CONTENT AND CLEAVAGED PRODUCT THEREOF

摘要

PROBLEM TO BE SOLVED: To provide a fraction having high milk-derived basic cystatin content and cleavaged products thereof, each suitable for use in food and drink, medicine and feed to prevent and ameliorate various kinds of osteopathy such as osteoporosis, osteoarthroses such as rheumatism and periodontal disease. SOLUTION: The subject method for producing a fraction of high milk-derived basic cystatin content comprises such processes that a basic protein fraction derived from milk is heated, if necessary, a supernatant obtained through removing thus produced precipitation from the resultant fraction is collected, an alcohol is added to the supernatant followed by collecting a fraction of high milk- derived basic cystatin content through removing thus produced precipitation therefrom, the fraction is contacted with a sulfuric acid group-containing carrier such as heparin followed by collecting a fraction which is not adsorbed to the carrier, the fraction is treated with an ultrafiltration membrane with a molecular weight cut off of 10-50 kDa to collect the filtrate. The other objective method for producing the cleavage product of the above fraction comprises splitting the above fraction with a protease.