Method of primer-extension preamplification PCR

摘要

The invention provides a method for the amplification of nucleic acid fragments from a sample that comprises two or three thermocyclic amplification reactions, whereby completely randomized primers are used in the first amplification reaction and specific primers are used in the second amplification reaction, characterized in that, to amplify the DNA, a mixture of at least two DNA polymerases is used, at least one of which possesses proofreading activity. Using this method, DNA from single cells or cell clones with a low cell count can be used in mutation analysis.