Improved method for primer extension preamplification-PCR

摘要

The invention relates to a method for amplification of nucleic acid fragments from a sample, which consists of two or three thermocycling amplification reactions, wherein during the first amplification reaction are used completely randomized primers and specific primers used in the second amplification reaction, which is characterized in that for the amplification of DNA is a mixture of at least two DNA polymerases is used, of which at least one polymerase having proofreading activity. In this manner, DNA can be obtained from single cells or cell clones used with a low number of cells for mutation analysis.