FLUORESCENT CAPTURE ASSAY FOR KINASE ACTIVITY EMPLOYING ANTI-PHOSPHOTYROSINE ANTIBODIES AS CAPTURE AND DETECTION AGENTS

摘要

A method for determining the level of tyrosine kinase activity in a biological sample is disclosed. The method employs an anti-phosphotyrosine antibody as both the capture agent and the detecting agent. The detecting antibody is labeled with a lanthanide ion, such as europium, as the signal generating entity. The method is particularly well suited to high throughput screening, for example, for compounds which modulate tyrosine kinase activity.