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Fluorescent capture assay for kinase activity employing anti-phosphotyrosine antibodies as capture and detection agents
Fluorescent capture assay for kinase activity employing anti-phosphotyrosine antibodies as capture and detection agents
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机译:使用抗磷酸酪氨酸抗体作为捕获和检测试剂的激酶活性的荧光捕获测定
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摘要
A method for determining the level of tyrosine kinase activity in a biological sample is disclosed. The method employs an anti-phosphotyrosine antibody as both the capture agent and the detecting agent. The detecting antibody is labeled with a lanthanide ion, such as europium, as the signal generating entity. The method is particularly well suited to high throughput screening, for example, for compounds which modulate tyrosine kinase activity.
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