Hepatitis C nucleic acid, recombinant vector containing it, oligonucleotides and their use, and method and test packages for amplification and detection of nucleic acid and isolated hepatitis C virus

摘要

The invention provides reagents, methods and kits for amplifying and detecting U.S., Japan, and HCV-C prototype nucleic acids.Oligonucleotides primers can be used to amplify and detect Hepatitis C virus nucleic acids in a process that involves reverse transcription of the viral genomic RNA to create cDNA and the subsequent amplification of the cDNA by the polymerase chain reaction. Oligonucleotide probes can be used to detect the presence of amplified DNA by hybridization.