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NUCLEIC ACID CONSTRUCT AND METHOD FOR IDENTIFYING THE EXPRESSION OF HETEROLOGOUS GENE IN A PLANT
NUCLEIC ACID CONSTRUCT AND METHOD FOR IDENTIFYING THE EXPRESSION OF HETEROLOGOUS GENE IN A PLANT
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机译:核糖核酸结构及鉴定植物中杂菌基因表达的方法
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摘要
The invention relates to a nucleic acid construct which comprises an AHAS promoter for expressing the gene in plants operationally linked to a sequence encoding a heterologous gene, a promoter which can be selected from the promoters of als 1 and als 2 genes from maize or AHAS promoter comprising SEQ.ID No. 1, SEQ ID No 2, or SEQ. ID No 3 sequence as well as to a method for identifying the gene expression in a plant and /or different tissues thereof and which consists in recombinantly transforming a portion of the plant, expressing the heterologous gene in the so transformed portion of the plant and detecting the expression of the gene.
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机译:本发明涉及一种核酸构建体,其包含用于在植物中表达该基因的AHAS启动子,该启动子与编码异源基因的序列可操作地连接,该启动子可以选自玉米的ALS 1和ALS 2基因的启动子或AHAS启动子。包含SEQ.ID No.1,SEQ ID No 2或SEQ。 ID No 3序列以及用于鉴定在植物和/或其不同组织中的基因表达的方法,其在于重组转化植物的一部分,在如此转化的植物的部分中表达异源基因并检测基因的表达。
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