INSECT VIRUS WITH REDUCED ABILITY TO EXPAND IN HABITAT FROM HOST TO HOST AND ITS PRODUCTION METHOD

摘要

An insect virus with a reduced ability to spread in the environment from host to host is described against insects by non-infectious alteration of the p74 gene encoding the 74 kDa protein of this virus, the infectivity of which is restored by complementation with the functional p74 gene or encoded by the p74 protein of the wild-type virus. , wherein the insect cell carrying the altered insect virus is additionally transfected with a DNA fragment providing the missing product or function of the altered p74 site of a functional wild-type virus gene. Also described is a method of making the virus comprising the steps of: a) depleting the virus of insect infectivity by altering the p74 site of the virus gene and b) restoring the virus's infectivity by complementing the altered virus with a product or function lacking or defective in the altered virus by transfecting an insect cell carrying this altered virus is a DNA fragment providing a product or function that is missing or defective at the p74 site of the wild-type gene.