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EXBB-EXBD-TONB GENE CLUSTER OF PSEUDOMONAS PUTIDA DOT-T1E CONFERRING TOLERANCE TO AROMATIC COMPOUNDS AND RESISTANCE TO ANTIBIOTICS
EXBB-EXBD-TONB GENE CLUSTER OF PSEUDOMONAS PUTIDA DOT-T1E CONFERRING TOLERANCE TO AROMATIC COMPOUNDS AND RESISTANCE TO ANTIBIOTICS
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机译:假单胞菌DOT-T1E的EXBB-EXBD-TONB基因簇赋予对芳香族化合物的耐受性和抗药性
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摘要
This invention relates to the isolation of a novel tonB operon from Pseudomonas putida. These genes are useful to render the cells more sensitive to antibiotics, toluene, pHBA, aromatic compounds, parabenes, and aromatic amino acids after inactivation with specific mutant allels or more tolerant to these compounds after overexpression with appropriate expression vector. These findings are important in the field of medicine and biotechnology and biocatalysis. In addition a screen to identify pHBA tolerant genes is provided and strains with significant tolerance to pHBA were identified. These strains are important for pHBA production.
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