A process for the production of 2-hydroxyphenylacetic acid using mutant bacteria pseudomonas putida or the well as a recombinant E. coli strain in which introduced genes isolated pseudomonas putida.

摘要

A process for the production of 2- hydroxyphenylacetic acid using mutant bacterium Pseudomonas putida U and a recombinant E. coli strain in which introduced genes isolated from Pseudomonas putida. The invention presented describes a process for the biotransformation of phenylacetic acid (PA) in 2-hydroxyphenylacetic acid (abbreviated indicated as 2-HPA), using mutants of the bacterium Pseudomonas putida U interrupted in one of the genes (phal) that catabolic pathway include phenylacetic acid, and the use of a strain of recombinant E. coli in which they have been introduced, and Pseudomonas putida los.genes isolates encoding the enzymes responsible for the biotransformation are expressed.